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The vitrification process leads to mitochondrial dysfunction, characterized by a reduction in mitochondrial membrane potential (MMP), an increase in ROS, and elevated mitochondrial Ca 2+ levels ( Mechanistically, oxidative stress compromises mitochondrial integrity primarily through the activation of the ASK1JNKBcl-2/Bax apoptotic axis

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Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

This version delivers double the dose per vial, which some practitioners prefer for patients seeking more noticeable brightening results

Data are representative of 3 independent experiments s.d